Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver

doi: 10.1073/pnas.1319388111

Figure Lengend Snippet: Uptake and transport of silver in C. reinhardtii. Intracellular concentrations of silver (A) on exposure to 100 and 200 nM, and (Inset) to 500-nM exposure. No intracellular silver was quantifiable in C. reinhardtii exposed to 10 nM silver and is therefore not shown in the graph. Heat map of Cu transporters (B) in C. reinhardtii exposed to silver for varying durations with each box representing a protein at the transcriptome and proteome level, green being down-regulated and red up-regulated. The SD is shown in the figures. Atx1, antioxidant 1, copper chaperone; Cox2A, subunit 2A of cytochrome oxidase; Ctr1 and -3, copper transporter 1 and 3; Fox1, Ferroxidase; Pcy1, Plastocyanin. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).

Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Techniques: Algae

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver

doi: 10.1073/pnas.1319388111

Figure Lengend Snippet: Regulation of functional pathways in C. reinhardtii at physiological (A, C, E, G) and molecular (B, D, F, H) levels. Photosynthesis (A, B); lipid peroxidation compared with the control (C) and oxidative stress response (D); growth (E, F); ATP content (G) and synthesis (H). In the heat maps each square represents a protein, with green being down-regulated and red up-regulated. For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).

Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Techniques: Functional Assay, Control, Algae

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver

doi: 10.1073/pnas.1319388111

Figure Lengend Snippet: Molecular and physiological changes of lipid synthesis in C. reinhardtii exposed to silver for 1 h. Regulation of proteins (A) and lipid bodies containing precursors of lipids stained green with Nile red (B). The chloroplasts autoflourescence is seen as red. (Scale bars in B, 10 µm.) For the molecular responses, algae exposed to 500 nM Ag+ were not analyzed (see Methods).

Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Techniques: Staining, Algae

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver

doi: 10.1073/pnas.1319388111

Figure Lengend Snippet: The toxicity and adaptive response pathways, as derived from linking transcriptome and proteome responses to physiological effects. (A) Schematic representation of biological pathways in C. reinhardtii affected by Ag+. (B) Schematic representation of the toxicity pathway. (C) Schematic representation of the adaptive-response pathway.

Article Snippet: A C. reinhardtii whole-genome gene-expression microarray (Genotypic Technology) was designed using the JGI V4 Chlamydomonas best-transcripts database.

Techniques: Derivative Assay